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Wild-type blocking polymerase chain reaction for detection of single
Aug 22, 2005 . Wild-type blocking polymerase chain reaction for detection of single nucleotide minority mutations from clinical specimens. Patrick L .

Laboratory Investigation - Different Applications of Polymerases With ...
With the completion of the human genome project, single-nucleotide . ability of exo+ polymerases was further evaluated in primer extension using three types of .

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A Single-Molecule Nanopore Device Detects DNA Polymerase ...
Jan 1, 2008 . Figure 3 Monitoring DNA polymerase-catalyzed single-nucleotide . of different starting lengths and two different types of DNA polymerase.

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The Base Substitution Fidelity of DNA Polymerase ?-dependent Single
May 6, 2003 . Single nucleotide BER requires several enzymatic activities, including . In comparison to wild type pol ?, these polymerases were previously .

Malaria Journal | Full text | IMP PCR primers detect single nucleotide ...
Polymerase chain reactions to distinguish single-nucleotide polymorphisms are . and Savanna rDNA types, and resistance to dieldrin in Anopheles arabiensis .

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DNA sequencing - Wikipedia, the free encyclopedia
RNA sequencing was one of the earliest forms of nucleotide sequencing. . method requires a single-stranded DNA template, a DNA primer, a DNA polymerase, normal . Four types of reversible terminator bases (RT-bases) are added, and .

Single-Nucleotide Polymorphism Identification Assays Using a ...
Single-Nucleotide Polymorphism Identification Assays Using a Thermostable DNA Polymerase and Delayed Extraction MALDI-TOF Mass Spectrometry .

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Eukaryotic DNA Polymerases
Polymerases Involved in DNA Repair. Many types of DNA repair involve removal of damaged or mismatched nucleotides to form a single-stranded region, .

Nucleotide sequence and polymerase chain reaction/restriction ...
by a single nucleotide, producing an amino acid alteration. Compared with other types of ADV, these isolates had 96% sequence similarity to a published ferret .

Nucleotide excision repair
However, the amount and types of mutations that accumulate in a genome are . Mutations commonly are substitutions, in which a single nucleotide is changed into a . It is estimated that E. coli DNA polymerase III holoenzyme (with a fully .

MALDI-TOF mass spectrometric typing of single nucleotide ...
Single nucleotide polymorphisms (SNPs) are the most common type of genetic variation in the eukaryotic genome. There is about one SNP per kb in . ddA, the polymerase extension MALDI-TOFMS procedure described above was unable to .

Codon Substitution Mutations at Two Positions in the L Polymerase ...
This single nucleotide change restored the ability of Y942H-p6 to replicate at restrictive temperatures, rendering it indistinguishable from the rHPIV1 wild-type .

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types of single nucleotide polymerases

Exo+ proofreading polymerases mediate genetic analysis and its ...
primer extension is a single nucleotide polymorphism (SNP) assay consisting of 3 . et al[6,7,14], four types of DNA polymerases, T4+, T7+, KF+ and Vent, were .

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Protection against Methylation-induced Cytotoxicity by DNA ...
MX exposure sensitizes wild-type, but not ?-pol null mouse embryonic fibroblasts, to the . For single-nucleotide repair, ?-pol is the polymerase of choice for the .

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